One person was killed in a two-vehicle crash north of Milverton on Wednesday night.
Emergency crews were called after the vehicles collided on Perth Line 72 between Road 147 and Road 136.
One person was pronounced dead at the scene, while two others were taken to hospital for treatment of non-life threatening injuries, Perth OPP said.
The identities of those involved have not been released.
The section of Perth Line 72 as well as Road 140 between Perth Line 71 and Perth Line 75 were closed until early Thursday morning.
Police are asking anyone with dash-camera footage of the collision to call investigators.