Blackfriars Bridge will be closed to vehicle traffic from Tuesday until Friday.
According to the city, the closure will allow for finishing touches to the bridge.
Pedestrians will still be able to cross the river using the pedestrian walkway.
The bridge re-opened in November 2018 after an $8-million project that saw the bridge cut in two and removed from its perch above the Thames River in 2017.
Built in 1875, it is one of the oldest and rarest bridges in Canada. Since then the wrought iron bowstring arch-truss bridge has spanned the north branch of the Thames River, connecting Ridout St. to Blackfriars St.
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With files from Miranda Chant